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Matrisome Biology Seminar - M. Florencia Sánchez

M. Florencia Sánchez

Biochemistry
Goethe University Frankfurt, Max

Ligand-free in situ confinement for GPCR activation and signal transduction
Wann 15.12.2021
von 13:00 bis 14:30
Wo Zoom-Meeting
Name
Teilnehmer Universitätsoffen / open to university members
Termin übernehmen vCal
iCal

Ligand-free in situ confinement for GPCR activation and signal transduction

 

Cell-cell communication and signal transduction rely on the assembly of receptorlig and complexes at the plasma membrane. The spatiotemporal receptor organization plays a pivotal role in evoking cellular responses. Nevertheless, the mechanism for cluster formation and how their localization within the plasma membrane influence cell responses is not comprehensively understood. Thus, traceless modifications with high spatiotemporal control that allow fine tuning of protein networks in a fast, reversible, and non-invasive manner are required. Here, by establishing modular matrices with a multivalent high-affinity nanotool, we rewired in situ the lateral membrane organization of the hormone neuropeptide Y2 receptor in living cells by light. Within seconds, receptor clustering can be modulated in size, location, and density. By in situ confinement, changes in cellular morphology, motility, and calcium signaling revealed a ligand-independent receptor activation. Overall, the matrix as well as the employed nanotool represent versatile instruments for tracking of cellular processes and depict now the possibility to elucidate unexplored mechanisms in cell signaling and mechanotransduction.

 

Dieses Seminar wird von der interdisziplinären FRIAS-Forschungsgruppe MatrixCode: matrisome pathology organisiert.

Dies ist eine Zoom-Veranstaltung. Wir bitten um eine