Robert Tampé: "Optochemical Biology- In-situ Assembly of Macromolecular Complexes Triggered by Light"

Robert Tampé
University of Frankfurt, Germany

Optochemical Biology - In-situ Assembly of Macromolecular Complexes Triggered by Light

Chemical and synthetic biology aims for a perfect control of cellular networks in time and space by site-specific labeling, manipulation, and structured organization of protein complexes. This seminar will summarize recent developments using generic interaction pairs, which can be triggered by light. Multiplexed assembly of protein complexes is realized by iterative in situ activation, writing and binding processes via scanning laser microscopy. These light-triggered recognition processes allow for a spatiotemporal control of protein-protein interactions and rewiring cellular pathways. Approaches how to trigger protein complexes in three dimensions are discussed.

1. Laboria N, Wieneke R, Tampé R (2013) Nanomolar interaction and in-situ assembly of proteins in four dimensions controlled by light. Angew Chem Int Ed, 52, 848-53.
2. Gatterdam V, Stoess T, Menge CM, Heckel A, Tampé R (2012) Caged glutathione – triggering protein interaction by light. Angew Chem Int Ed 51, 3726-3729.
3. Grunwald C, Schulze K, Giannone G, Cognet L, Lounis B, Choquet D, Tampé R (2011) Quantum yield optimized fluorophores for site-specific labeling and super-resolution imaging. J Am Chem Soc 133, 8090-8093.
4. Kleefen A, Pedone D, Grunwald C, Wei R, Firnkes M, Abstreiter G, Rant U, Tampé R (2010) Multiplexed parallel single transport recordings on nanopore arrays. Nano Lett 10, 5080-5087.
5. Grunwald C, Schulze K, Reichel A, Weiss VU, Piehler J, Wiesmüller KH, Tampé R (2010) In situ assembly of macromolecular complexes triggered by light. Proc Natl Acad Sci USA 107, 6146-6151.