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Publikationen Natur- und Ingenieurwissenschaften 2015
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Originalarbeiten in wissenschaftlichen Fachzeitschriften Jahre: 2015 | alle anzeigen zurück zur Übersicht aller Publikationen Moazenzadeh A , Sandoval F , Spengler N , Badilita V , Wallrabe U 3-D Microtransformers for DC-DC On-Chip Power Conversion 2015 IEEE TRANSACTIONS ON POWER ELECTRONICS , Band : 30, Nummer : 9, Seiten : 5088 - 5102» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung We address the miniaturization of power converters by introducing novel 3-D microtransformers with magnetic core for low-megahertz frequency applications. The core is fabricated by lamination and microstructuring of Metglas 2714A magnetic alloy. The solenoids of the microtransformers are wound around the core using a ball-wedge wirebonder. The wirebonding process is fast, allowing the fabrication of solenoids with up to 40 turns in 10 s. The fabricated devices yield the high inductance per unit volume of 2.95 mu H/mm(3) and energy per unit volume of 133 nJ/mm(3) at the frequency of 1 MHz. The power efficiency of 64-76% is measured for different turns ratio with coupling factors as high as 98%. To demonstrate the applicability of our passive components, two PWM controllers were selected to implement an isolated and a nonisolated switch-mode power supply. The isolated converter operates with overall efficiency of 55% and maximum output power of 136 mW; then, we experimentally demonstrate how we increased this efficiency to 71% and output power to 408 mW. The nonisolated converter can deliver an overall efficiency of 81% with a maximum output power of 515 mW. Finally, we benchmarked the results to underline the potential of the technology for power on-chip applications.Liu Z , Boachon B , ... , Werck-Reichhart D , Renault H A Conserved Cytochrome P450 Evolved in Seed Plants Regulates Flower Maturation 2015 MOLECULAR PLANT , Band : 8, Nummer : 12, Seiten : 1751 - 1765» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Global inspection of plant genomes identifies genes maintained in low copies across taxa and under strong purifying selection, which are likely to have essential functions. Based on this rationale, we investigated the function of the low-duplicated CYP715 cytochrome P450 gene family that appeared early in seed plants and evolved under strong negative selection. Arabidopsis CYP715A1 showed a restricted tissue-specific expression in the tapetum of flower buds and in the anther filaments upon anthesis. cyp715a1 insertion lines showed a strong defect in petal development, and transient alteration of pollen intine deposition. Comparative expression analysis revealed the downregulated expression of genes involved in pollen development, cell wall biogenesis, hormone homeostasis, and floral sesquiterpene biosynthesis, especially TPS21 and several key genes regulating floral development such as MYB21, MYB24, and MYC2. Accordingly, floral sesquiterpene emission was suppressed in the cyp715a1 mutants. Flower hormone profiling, in addition, indicated a modification of gibberellin homeostasis and a strong disturbance of the turnover of jasmonic acid derivatives. Petal growth was partially restored by the active gibberellin GA(3) or the functional analog of jasmonoyl-isoleucine, coronatine. CYP715 appears to function as a key regulator of flower maturation, synchronizing petal expansion and volatile emission. It is thus expected to be an important determinant of flower-insect interaction.Feng L , Korvink JG , Benner P A Fully Adaptive Scheme for Model Order Reduction Based on Moment Matching 2015 IEEE TRANSACTIONS ON COMPONENTS PACKAGING AND MANUFACTURING TECHNOLOGY , Band : 5, Nummer : 12, Seiten : 1872 - 1884» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung A fully adaptive model order reduction scheme based on moment matching is proposed to derive the reduced-order models of linear time-invariant (LTI) systems. According to the given error tolerance, the order of the reduced-order model as well as the expansion points for the transfer function is automatically determined on the fly during the process of model reduction. In this sense, the reduced-order model is automatically obtained without assigning the number of moments and expansion points in a priori, which is a prerequisite for the standard implementation of model reduction based on moment matching. The proposed adaptive scheme is found to be efficient when it is tested on various LTI systems.Cazade PA , Zheng W , Prada-Gracia D , Berezovska G , Rao F , Clementi C , Meuwly M A comparative analysis of clustering algorithms: O2 migration in truncated hemoglobin I from transition networks. 2015 J Chem Phys , Band : 142, Nummer : 2, Seiten : 025103 - 025103Boehler C , Asplund M A detailed insight into drug delivery from PEDOT based on analytical methods: effects and side effects. 2015 J Biomed Mater Res A , Band : 103, Nummer : 3, Seiten : 1200 - 1207Weise M , ... , Aktories K , Orth J A systemic Pasteurella multocida toxin aggravates cardiac hypertrophy and fibrosis in mice 2015 CELLULAR MICROBIOLOGY , Band : 17, Nummer : 9, Seiten : 1320 - 1331Braun RJ , Sommer C , Leibiger C , Gentier RJ , Dumit VI , ... , Dengjel J , van Leeuwen FW , Kroemer G , Madeo F Accumulation of Basic Amino Acids at Mitochondria Dictates the Cytotoxicity of Aberrant Ubiquitin. 2015 Cell Rep Heizmann S , ... , Boehler C , Ruther P , Egert U , Asplund M Accurate neuronal tracing of microelectrodes based on PEDOT-dye coatings 2015 Neural Engineering (NER), 2015 7th International IEEE/EMBS Conference , Seiten : 386 - 389» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Penetrating microelectrode probes offer the opportunity to precisely monitor neuronal signals over several months. After the experimental end point, histological methods are used to analyze the tissue and to correlate recorded signals to histological findings. However, accurate retroactive tracing of the position of individual microelectrodes is currently hampered by the lack of suitable techniques to mark their positions. Here we describe a poly (3, 4-ethylene dioxythiophene) (PEDOT) based microelectrode coating, which is optimized for an active, electrochemically controllable release of the neuronal tracer DiI. The tracer is incorporated into the polymer coating by an ion exchange mechanism. Active cycling showed a 3.6 times higher dye incorporation rate compared to reference samples which were solely immersed in the dye. A bi-layer system was used to optimize drug storage ability and could suppress passive leakage of DiI from the film by more than a factor 6. The PEDOT/dye system was characterized in vitro in terms of its ability to store the dye over the time course of the experiment, deliver a precise quantity upon electroactivation and continuously support stable recordings throughout an implantation.Orellana-Escobedo L , Rosales-Mendoza S , ... , Reski R An Env-derived multi-epitope HIV chimeric protein produced in the moss Physcomitrella patens is immunogenic in mice 2015 PLANT CELL REPORTS , Band : 34, Nummer : 3, Seiten : 425 - 433» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The first report on the recombinant production of a candidate vaccine in the moss system. The need for economical and efficient platforms for vaccine production demands the exploration of emerging host organisms. In this study, the production of an antigenic protein is reported employing the moss Physcomitrella patens as an expression host. A multi-epitope protein from the Human Immunodeficiency Virus (HIV) based on epitopes from gp120 and gp41 was designed as a candidate subunit vaccine and named poly-HIV. Transgenic moss plants were generated carrying the corresponding poly-HIV transgene under a novel moss promoter and subsequently seven positive lines were confirmed by PCR. The poly-HIV protein accumulated up to 3.7 A mu g g(-1) fresh weight in protonema cultures. Antigenic and immunogenic properties of the moss-produced recombinant poly-HIV are evidenced by Western blots and by mice immunization assays. The elicitation of specific antibodies in mice was observed, reflecting the immunogenic potential of this moss-derived HIV antigen. This is the first report on the production of a potential vaccine in the moss system and opens the avenue for glycoengineering approaches for the production of HIV human-like glycosylated antigens as well as other vaccine prototypes under GMP conditions in moss bioreactors.Zou P , Laird D , ... , Lienkamp K Antimicrobial and cell-compatible surface-attached polymer networks - how the correlation of chemical structure to physical and biological data leads to a modified mechanism of action 2015 JOURNAL OF MATERIALS CHEMISTRY B , Band : 3, Nummer : 30, Seiten : 6224 - 6238» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung We present a synthetic platform based on photo-induced thiol-ene chemistry, by which surface-attached networks from antimicrobial poly(oxonorbornene) (so-called polymeric synthetic mimics of antimicrobial peptides, SMAMPs) could be easily obtained. By systematically varying hydrophobicity and charge density, surface-attached polymer networks with high antimicrobial activity and excellent cell compatibility were obtained. For the homopolymer networks with constant charge density, antimicrobial activity increased systematically with increasing hydrophobicity (i.e. decreasing swellability and apparent surface energy). Irrespective of charge density, the antimicrobial activity of all networks correlated with the acid constant pK and the isoelectric point (IEP) - the lower pK and IEP, the higher the antimicrobial activity. The cell compatibility of the networks increased with increasing swellability and apparent surface energy, and decreased with increasing charge density. The data corroborates that the mechanism of action of antimicrobial polymer surfaces depends on at least two mechanistic steps, one of which is hydrophobicity-driven and the other charge related. Therefore, we suggest a modified mechanistic model with a charge-driven and a hydrophobicity-driven step. For antimicrobial networks that only varied in hydrophobicity, the antimicrobial activities on surfaces and in solution also correlated - the higher the activity in solution, the higher the activity on surfaces. Thus, the hydrophobicity-driven step for activity on surfaces may be similar to the one in solution. Cell compatibility of SMAMPs in solution and on surfaces also showed a systematic positive correlation for all polymers, therefore this property also depends on the net hydrophobic balance of the polymer.Baluschev S Assembly of New Merocyanine Chromophores with a 1,8-Naphthalimide Core by a New Method for the Synthesis of the Methine Function 2015 Aust J Chem , Band : 68, Seiten : 1399 - 1408Sommerlade L , Thiel M , Mader M , Mader W , Timmer J , Platt B , Schelter B Assessing the strength of directed influences among neural signals: an approach to noisy data. 2015 J Neurosci Meth , Band : 239, Seiten : 47 - 64Leitner DM , Pandey HD Asymmetric energy flow in liquid alkylbenzenes: A computational study 2015 J Chem Phys , Band : 143Johnson GR , Li J , Shariff A , Rohde GK , Murphy RF Automated Learning of Subcellular Variation among Punctate Protein Patterns and a Generative Model of Their Relation to Microtubules. 2015 Plos Comput Biol , Band : 11, Nummer : 12, Seiten : e1004614 - e1004614Mullineaux CW Bacteria in solitary confinement. 2015 J Bacteriol , Band : 197, Nummer : 4, Seiten : 670 - 671Jank T , Belyi Y , Aktories K Bacterial glycosyltransferase toxins. 2015 Cell Microbiol , Band : 17, Nummer : 12, Seiten : 1752 - 1765Rastogi V , Samyn P Bio-Based Coatings for Paper Applications 2015 COATINGS , Band : 5, Nummer : 4, Seiten : 887 - 930» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The barrier resistance and wettability of papers are commonly controlled by the application of petroleum-based derivatives such as polyethylene, waxes and/or fluor-derivatives as coating. While surface hydrophobicity is improved by employing these polymers, they have become disfavored due to limitations in fossil-oil resources, poor recyclability, and environmental concerns on generated waste with lack of biodegradation. Alternatively, biopolymers including polysaccharides, proteins, lipids and polyesters can be used to formulate new pathways for fully bio-based paper coatings. However, difficulties in processing of most biopolymers may arise due to hydrophilicity, crystallization behavior, brittleness or melt instabilities that hinder a full exploitation at industrial scale. Therefore, blending with other biopolymers, plasticizers and compatibilizers is advantageous to improve the coating performance. In this paper, an overview of barrier properties and processing of bio-based polymers and their composites as paper coating will be discussed. In particular, recent technical advances in nanotechnological routes for bio-based nano-composite coatings will be summarized, including the use of biopolymer nanoparticles, or nanofillers such as nanoclay and nanocellulose. The combination of biopolymers along with surface modification of nanofillers can be used to create hierarchical structures that enhance hydrophobicity, complete barrier protection and functionalities of coated papers.Mueller SJ , Gütle DD , Jacquot J-P , Reski R Can mosses serve as model organisms for forest research? 2015 Ann Forest Sci Ribeiro S , Buhmann S , Stielow T , Scheel S Casimir-Polder interaction from exact diagonalization and surface-induced state mixing 2015 EPL , Band : 110, Nummer : 5» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Dispersion forces have a sizeable effect on the energy levels of highly excited Rydberg atoms when brought close to material surfaces. Rydberg atoms experience energy shifts in the GHz range at micrometer distances, suggestive of considerable state admixture. We show that despite the non-applicability of perturbation theory for Rydberg atoms near a surface, the energy shift due to the dispersion interaction can be obtained from an exact diagonalization of the interaction Hamiltonian by finding the zeros of the Pick function. Moreover, we show that contrary to intuition from single-mode approaches, surface-induced state mixing is generally suppressed even for large interaction energies. We give a tailored example where mixing is observable despite this effect. Copyright (C) EPLA, 2015Lichtenthaler MR , Stahl F , Kratzert D , Heidinger L , Schleicher E , Hamann J , Himmel D , Weber S , Krossing I Cationic cluster formation versus disproportionation of low-valent indium and gallium complexes of 2,2'-bipyridine. 2015 Nat Commun , Band : 6, Seiten : 8288 - 8288Güven ÜB , Kegel OH , Kuzucuoglu M Centralizers of Subgroups in Direct Limits of Symmetric Groups with Strictly Diagonal Embedding 2015 Commun Algebra , Band : 43, Nummer : 5, Seiten : 1920 - 1934Beratan DN , Liu C , Migliore A , Polizzi NF , Skourtis SS , Zhang P , Zhang Y Charge transfer in dynamical biosystems, or the treachery of (static) images. 2015 Accounts Chem Res , Band : 48, Nummer : 2, Seiten : 474 - 481Beike AK , Spagnuolo V , ... , Decker EL , Reski R Clonal in vitro propagation of peat mosses (Sphagnum L.) as novel green resources for basic and applied research. 2015 Plant Cell Tiss Org , Band : 120, Nummer : 3, Seiten : 1037 - 1049Moilanen JM , Kokkonen N , Loffek S , Vayrynen JP , Syvaniemi E , Hurskainen T , Makinen M , Klintrup K , Makela J , Sormunen R , Bruckner-Tuderman L , Autio-Harmainen H , Tasanen K Collagen XVII expression correlates with the invasion and metastasis of colorectal cancer. 2015 Hum Pathol , Band : 46, Nummer : 3, Seiten : 434 - 442Haddadfarshi F , Cui J , Mintert F Completely positive approximate solutions of driven open quantum systems. 2015 Phys Rev Lett , Band : 114, Nummer : 13, Seiten : 130402 - 130402Nazeer H , Nguyen M , ... , Elwenspoek M Compositional Dependence of the Young’s Modulus and Piezoelectric Coefficient of (110)-Oriented Pulsed Laser Deposited PZT Thin Films 2015 JOURNAL OF MICROELECTROMECHANICAL SYSTEMS , Band : 24, Nummer : 1, Seiten : 166 - 173» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung In this contribution, we report on the compositional dependence of the mechanical and piezoelectric properties of Pb(ZrxTi1-x)O-3 (PZT) thin films fabricated by pulsed laser deposition (PLD). These films grow epitaxially on silicon with a (110) preferred orientation and have excellent piezoelectric properties, which make them outstanding candidates for application in microelectromechanical system devices. Vibrometric measurements on capacitors showed that the effective longitudinal piezoelectric coefficient (d(33,f)) of 100-nm thick PZT films has a maximum value of 72 pm/V for a composition of x = 0.52. The Young’s modulus was determined by measuring the difference in the flexural resonance frequencies of cantilevers before and after the deposition of the PZT thin films. The compositional dependence of the Young’s modulus shows an increase in value for the Zr-rich compositions, which is in agreement with the trend observed in their bulk ceramic counterparts. From the obtained dielectric constant and d(33,f), we show that the calculated coupling coefficients of the PLD-PZT thin films have higher values for most of the compositions than their ceramic counterparts. [2013-0039]von Prillwitz K , Rudnicki Ł , Mintert F Contrast in Multipath Interference and Quantum Coherence 2015 Phys Rev A , Band : 92, Seite : 052114Yaari R , Noy‑Malka C , Wiedemann G , Auerbach Gershovitz N , Reski R , Katz A , Ohad N DNA METHYLTRANSFERASE 1 is involved in mCG and mCCG
DNA methylation and is essential for sporophyte development
in Physcomitrella patens 2015 Plant Mol Biol Brandstetter-Kunc A , Weick G , Weinmann D , Jalabert R Decay of dark and bright plasmonic modes in a metallic nanoparticle dimer 2015 Phys Rev B , Band : 91Temerinac-Ott M , Naik AW , Murphy RF Deciding when to stop: efficient experimentation to learn to predict drug-target interactions. 2015 Bmc Bioinformatics , Band : 16, Seiten : 213 - 213Hurskainen T , Kokkonen N , ... , Bruckner-Tuderman L , Tasanen K Deletion of the major bullous pemphigoid epitope region of collagen XVII induces blistering, autoimmunization, and itching in mice. 2015 J Invest Dermatol , Band : 135, Nummer : 5, Seiten : 1303 - 1310Huber MC , Schreiber A , von Olshausen P , Varga BR , Kretz O , Joch B , Barnert S , Schubert R , Eimer S , Kele P , Schiller SM Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments. 2015 Nat Mater , Band : 14, Nummer : 1, Seiten : 125 - 132Scheel S , Buhmann S , Clausen C , Schneeweiss P Directional spontaneous emission and lateral Casimir-Polder force on an atom close to a nanofiber 2015 PHYSICAL REVIEW A , Band : 92, Nummer : 4, Seite : 043819» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung We study the spontaneous emission of an excited atom close to an optical nanofiber and the resulting scattering forces. For a suitably chosen orientation of the atomic dipole, the spontaneous emission pattern becomes asymmetric and a resonant Casimir-Polder force parallel to the fiber axis arises. For a simple model case, we show that such a lateral force is due to the interaction of the circularly oscillating atomic dipole moment with its image inside the material. With the Casimir-Polder energy being constant in the lateral direction, the predicted lateral force does not derive from a potential in the usual way. Our results have implications for optical force measurements on a substrate as well as for laser cooling of atoms in nanophotonic traps.Bae J Discrimination of two-qubit unitaries via local operations and classical communication 2015 Sci Rep-uk , Band : 5, Seite : 18720Grabert H Dynamical Coulomb blockade of tunnel junctions driven by alternating voltages 2015 PHYSICAL REVIEW B , Band : 92, Nummer : 24» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The theory of the dynamical Coulomb blockade is extended to tunneling elements driven by a time-dependent voltage. It is shown that, for standard setups where an external voltage is applied to a tunnel junction via an impedance, time-dependent driving entails an excitation of the modes of the electromagnetic environment by the applied voltage. Previous approaches for ac driven circuits need to be extended to account for the driven bath modes. A unitary transformation involving also the variables of the electromagnetic environment is introduced which allows us to split off the time dependence from the Hamiltonian in the absence of tunneling. This greatly simplifies perturbation-theoretical calculations based on treating the tunneling Hamiltonian as a perturbation. In particular, the average current flowing in the leads of the tunnel junction is studied. Explicit results are given for the case of an applied voltage with a constant dc part and a sinusoidal ac part. The connection with standard dynamical Coulomb blockade theory for constant applied voltage is established. It is shown that an alternating voltage source reveals significant additional effects caused by the electromagnetic environment. The hallmark of the dynamical Coulomb blockade in ac driven devices is a suppression of higher harmonics of the current by the electromagnetic environment. The theory presented basically applies to all tunneling devices driven by alternating voltages.Varatharajan R , ... , Hofmann U , Moser A , Tronnier V Electrical high frequency stimulation modulates GABAergic activity in the nucleus accumbens of freely moving rats 2015 NEUROCHEMISTRY INTERNATIONAL , Band : 90, Seiten : 255 - 260Crosse J , Fuchs S , Buhmann S Electromagnetic Green’s function for layered topological insulators 2015 PHYSICAL REVIEW A , Band : 92, Nummer : 6» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The dyadic Green’s function of the inhomogeneous vector Helmholtz equation describes the field pattern of a single frequency point source. It appears in the mathematical description of many areas of electromagnetism and optics including both classical and quantum, linear and nonlinear optics, dispersion forces (such as the Casimir and Casimir-Polder forces), and in the dynamics of trapped atoms and molecules. Here we compute the Green’s function for a layered topological insulator. Via the magnetoelectric effect, topological insulators are able to mix the electric E and magnetic induction B fields and, hence, one finds that the TE and TM polarizations mix on reflection from or transmission through an interface. This leads to unusual field patterns close to the surface of a topological insulator.Alexandrov A Enumerative Geometry, Tau-Functions and Heisenberg-Virasoro Algebra 2015 COMMUNICATIONS IN MATHEMATICAL PHYSICS , Band : 338, Nummer : 1, Seiten : 195 - 249» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung In this paper we establish relations between three enumerative geometry tau-functions, namely the Kontsevich-Witten, Hurwitz and Hodge tau-functions. The relations allow us to describe the tau-functions in terms of matrix integrals, Virasoro constraints and Kac-Schwarz operators. All constructed operators belong to the algebra (or group) of symmetries of the KP hierarchy.Zermiani M , Zonin E , ... , Palme K , Quintieri L , Ruperti B Ethylene negatively regulates transcript abundance of ROP-GAP rheostat-encoding genes and affects apoplastic reactive oxygen species homeostasis in epicarps of cold stored apple fruits 2015 JOURNAL OF EXPERIMENTAL BOTANY , Band : 66, Nummer : 22, Seiten : 7255 - 7270» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Apple (Malusxdomestica Borkh) fruits are stored for long periods of time at low temperatures (1 degrees C) leading to the occurrence of physiological disorders. ‘Superficial scald’ of Granny Smith apples, an economically important ethylene-dependent disorder, was used as a model to study relationships among ethylene action, the regulation of the ROP-GAP rheostat, and maintenance of H2O2 homeostasis in fruits during prolonged cold exposure. The ROP-GAP rheostat is a key module for adaptation to low oxygen in Arabidopsis through Respiratory Burst NADPH Oxidase Homologs (RBOH)-mediated and ROP GTPase-dependent regulation of reactive oxygen species (ROS) homeostasis. Here, it was shown that the transcriptional expression of several components of the apple ROP-GAP machinery, including genes encoding RBOHs, ROPs, and their ancillary proteins ROP-GEFs and ROP-GAPs, is coordinately and negatively regulated by ethylene in conjunction with the progressive impairment of apoplastic H2O2 homeostatic levels. RNA sequencing analyses showed that several components of the known ROP- and ROS-associated transcriptional networks are regulated along with the ROP-GAP rheostat in response to ethylene perception. These findings may extend the role of the ROP-GAP rheostat beyond hypoxic responses and suggest that it may be a functional regulatory node involved in the integration of ethylene and ROS signalling pathways in abiotic stress.Asl H , Talukdar H , ... , Michoel T , Bjorkegren J , Consortium CARDIoGRAM Expression Quantitative Trait Loci Acting Across Multiple Tissues Are Enriched in Inherited Risk for Coronary Artery Disease 2015 Circulation-Cardiovascular Genetics , Band : 8, Nummer : 2» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Background-Despite recent discoveries of new genetic risk factors, the majority of risk for coronary artery disease (CAD) remains elusive. As the most proximal sensor of DNA variation, RNA abundance can help identify subpopulations of genetic variants active in and across tissues mediating CAD risk through gene expression. Methods and Results-By generating new genomic data on DNA and RNA samples from the Stockholm Atherosclerosis Gene Expression (STAGE) study, 8156 cis-acting expression quantitative trait loci (eQTLs) for 6450 genes across 7 CAD-relevant tissues were detected. The inherited risk enrichments of tissue-defined sets of these eQTLs were assessed using 2 independent genome-wide association data sets. eQTLs acting across increasing numbers of tissues were found increasingly enriched for CAD risk and resided at regulatory hot spots. The risk enrichment of 42 eQTLs acting across 5 to 6 tissues was particularly high (<= 7.3-fold) and confirmed in the combined genome-wide association data from Coronary Artery Disease Genome Wide Replication And Meta-Analysis Consortium. Sixteen of the 42 eQTLs associated with 19 master regulatory genes and 29 downstream gene sets (n>30) were further risk enriched comparable to that of the 153 genome-wide association risk single-nucleotide polymorphisms established for CAD (8.4-fold versus 10-fold). Three gene sets, governed by the master regulators FLYWCH1, PSORSIC3, and G3BP1, segregated the STAGE patients according to extent of CAD, and small interfering RNA targeting of these master regulators affected cholesterol-ester accumulation in foam cells of the THP1 monocytic cell line. Conclusions-eQTLs acting across multiple tissues are significant carriers of inherited risk for CAD. FLYWCH1, PSORSIC3, and G3BP1 are novel master regulatory genes in CAD that may be suitable targets.Hieke N , ... , Dengjel J , Holland P , Simonsen A , Wesselborg S , Mizushima N , Stork B Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. 2015 Autophagy , Band : 11, Nummer : 9, Seiten : 1471 - 1483Zimmermann K , Lugan P , ... , Buchleitner A Extracting partial decay rates of helium from complex rotation: autoionizing resonances of the one-dimensional configurations 2015 JOURNAL OF PHYSICS B-ATOMIC MOLECULAR AND OPTICAL PHYSICS , Band : 48, Nummer : 2» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Partial autoionization rates of doubly excited one-dimensional helium in the collinear Zee and eZe configuration are obtained by means of the complex rotation method. The approach presented here relies on a projection of back-rotated resonance wave functions onto singly ionized He+ channel wave functions and the computation of the corresponding particle fluxes. In spite of the long-range nature of the Coulomb potential between the electrons and the nucleus, an asymptotic region where the fluxes are stationary is clearly observed. Low-lying doubly excited states are found to decay predomintantly into the nearest single-ionization continuum. This approach paves the way for a systematic analysis of the decay rates observed in higher-dimensional models, and of the role of electronic correlations and atomic structure in recent photoionization experiments.Koegel J , Linder T , Schroeder F , Sundermeyer J , Goll S , Himmel D , Krossing I , Kuett K , Saame J , Leito I Fluoro- and Perfluoralkylsulfonylpentafluoroanilides: Synthesis and Characterization of NH Acids for Weakly Coordinating Anions and Their Gas-Phase and Solution Acidities 2015 CHEMISTRY-A EUROPEAN JOURNAL , Band : 21, Nummer : 15, Seiten : 5769 - 5782» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Fluoro- and perfluoralkylsulfonyl pentafluoroanilides [HN(C6F5)(SO2X); X = F, CF3, C4F9, C8F17] are a class of imides with two different strongly electron-withdrawing sub-stituents attached to a nitrogen atom. They are NH acids, the unsymmetrical hybrids of the well-known symmetrical bissulfonylimides and bispentafluorophenylamine. The syntheses, the structures of these perfluoroanilides, their solvates, and some selected lithium salts give rise to a structural variety beyond the symmetrical parent compounds. The acidities of representative subsets of these novel NH acids have been investigated experimentally and quantum-chemically and their gas-phase acidities (GAs) are reported, as well as the pK(a) values of these compounds in acetonitrile (MeCN) and DMSO solution. In quantum chemical investigations with the vertical and relaxed COSMO cluster-continuum models (vCCC/rCCC), the unusual situation is encountered that the DMSO-solvated acid Me2SO-H-N(SO2CF3)(2), optimized in the gas phase (vCCC model), dissociates to Me2SO-H+ -N(SO2CF3)(2)(-) during structural relaxation and full optimization with the solvation model turned on (rCCC model). This proton transfer underlines the extremely high acidity of HN(SO2CF3)(2). The importance of this effect is studied computationally in DMSO and MeCN solution. Usually this effect is less pronounced in MeCN and is of higher importance in the more basic solvent DMSO. Nevertheless, the neglect of the structural relaxation upon solvation causes typical changes in the computational pK(a) values of 1 to 4 orders of magnitude (4-20 kJmol(-1)). The results provide evidence that the published experimental DMSO pK(a) value of HN(SO2CF3)(2) should rather be interpreted as the pK(a) of a Me2SO-H+-N(SO2CF3)(2)(-) contact ion pair.Intravaia F , Mkrtchian V , Buhmann S , Scheel S , Dalvit D , Henkel C Friction forces on atoms after acceleration 2015 JOURNAL OF PHYSICS-CONDENSED MATTER , Band : 27, Nummer : 21, SI» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The aim of this paper is to revisit the calculation of atom-surface quantum friction in the quantum field theory formulation put forward by Barton (2010 New J. Phys. 12 113045). We show that the power dissipated into field excitations and the associated friction force depend on how the atom is boosted from being initially at rest to a configuration in which it is moving at constant velocity (nu) parallel to the planar interface. In addition, we point out that there is a subtle cancellation between the one-photon and part of the two-photon dissipating power, resulting in a leading order contribution to the frictional power which goes as nu(4). These results are also confirmed by an alternative calculation of the average radiation force, which scales as nu(3).Decker E , Wiedemann G , Reski R Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors 2015 GLYCO-ENGINEERING: METHODS AND PROTOCOLS , Band : 1321, Seiten : 213 - 224» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung One of the main challenges for the production of biopharmaceuticals in plant-based systems is the modulation of plant-specific glycosylation patterns towards a humanized form. Posttranslational modifications in plants are similar to those in humans, but several differences affect product quality and efficacy and can also cause immune responses in patients. In the moss Physcomitrella patens highly efficient gene targeting via homologous recombination enables glyco-engineering to obtain suitable platform lines for the production of recombinant proteins and biopharmaceuticals. Here we describe the methods which are effective for creating gene targeting constructs and transgenic moss lines as well as confirming successful homologous integration of the constructs and modification of target gene expression.Buchenberg S , Schaudinnus N , Stock G Hierarchical Biomolecular Dynamics: Picosecond Hydrogen Bonding Regulates Microsecond Conformational Transitions. 2015 J Chem Theory Comput , Band : 11, Nummer : 3, Seiten : 1330 - 1336Gajer JM , Furdas SD , Grunder A , Gothwal M , Heinicke U , Keller K , Colland F , Fulda S , Pahl HL , Fichtner I , Sippl W , Jung M Histone acetyltransferase inhibitors block neuroblastoma cell growth in vivo. 2015 Oncogenesis , Band : 4, Seiten : e137 - e137Labi V , Erlacher M How cell death shapes cancer. 2015 Cell Death Dis , Band : 6, Seiten : e1675 - e1675Zermiani M , Begheldo M , Nonis A , Palme K , et al. Identification of the arabidopsis RAM/MOR signalling network: adding new regulatory players in plant stem cell maintenance and cell polarization 2015 ANNALS OF BOTANY , Band : 116, Nummer : 1, Seiten : 69 - 89» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Background and Aims The RAM/MOR signalling network of eukaryotes is a conserved regulatory module involved in co-ordination of stem cell maintenance, cell differentiation and polarity establishment. To date, no such signalling network has been identified in plants. Methods Genes encoding the bona fide core components of the RAM/MOR pathway were identified in Arabidopsis thaliana (arabidopsis) by sequence similarity searches conducted with the known components from other species. The transcriptional network(s) of the arabidopsis RAM/MOR signalling pathway were identified by running in-depth in silico analyses for genes co-regulated with the core components. In situ hybridization was used to confirm tissue-specific expression of selected RAM/MOR genes. Key Results Co-expression data suggested that the arabidopsis RAM/MOR pathway may include genes involved in floral transition, by co-operating with chromatin remodelling and mRNA processing/post-transcriptional gene silencing factors, and genes involved in the regulation of pollen tube polar growth. The RAM/MOR pathway may act upstream of the ROP1 machinery, affecting pollen tube polar growth, based on the co-expression of its components with ROP-GEFs. In silico tissue-specific co-expression data and in situ hybridization experiments suggest that different components of the arabidopsis RAM/MOR are expressed in the shoot apical meristem and inflorescence meristem and may be involved in the fine-tuning of stem cell maintenance and cell differentiation. Conclusions The arabidopsis RAM/MOR pathway may be part of the signalling cascade that converges in pollen tube polarized growth and in fine-tuning stem cell maintenance, differentiation and organ polarity.Figge MT , Murphy RF Image-based systems biology. 2015 Cytom Part A , Band : 87, Nummer : 6, Seiten : 459 - 461Dixit PD , Jain A , Stock G , Dill KA Inferring Transition Rates of Networks from Populations in Continuous-Time Markov Processes. 2015 J Chem Theory Comput , Band : 11, Nummer : 11, Seiten : 5464 - 5472Beike A , Lang D , ... , Decker E , Reski R Insights from the cold transcriptome of Physcomitrella patens: global specialization pattern of conserved transcriptional regulators and identification of orphan genes involved in cold acclimation 2015 NEW PHYTOLOGIST , Band : 205, Nummer : 2, Seiten : 869 - 881» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The whole-genome transcriptomic cold stress response of the moss Physcomitrella patens was analyzed and correlated with phenotypic and metabolic changes. Based on time-series microarray experiments and quantitative real-time polymerase chain reaction, we characterized the transcriptomic changes related to early stress signaling and the initiation of cold acclimation. Transcription-associated protein (TAP)-encoding genes of P.patens and Arabidopsis thaliana were classified using generalized linear models. Physiological responses were monitored with pulse-amplitude-modulated fluorometry, high-performance liquid chromatography and targeted high-performance mass spectrometry. The transcript levels of 3220 genes were significantly affected by cold. Comparative classification revealed a global specialization of TAP families, a transcript accumulation of transcriptional regulators of the stimulus/stress response and a transcript decline of developmental regulators. Although transcripts of the intermediate to later response are from evolutionarily conserved genes, the early response is dominated by species-specific genes. These orphan genes may encode as yet unknown acclimation processes.Hemmasi S , Czulkies B , ... , Aktories K , Papatheodorou P Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR) 2015 JOURNAL OF BIOLOGICAL CHEMISTRY , Band : 290, Nummer : 22, Seiten : 14031 - 14044Filatov MA , Etzold F , Gehrig D , Laquai F , Busko D , Landfester K , Baluschev S Interplay Between Singlet and Triplet Excited States in a Conformationally Locked Donor-Acceptor Dyad 2015 Dalton T Jank T , ... , Aktories K Intracellular plasma membrane guidance of Photorhabdus asymbiotica toxin is crucial for cell toxicity 2015 FASEB JOURNAL , Band : 29, Nummer : 7, Seiten : 2789 - 2802Matsakas A , Prosdocimo DA , Mitchell R , Collins-Hooper H , Giallourou N , Swann JR , Potter P , Epting T , Jain MK , Patel K Investigating mechanisms underpinning the detrimental impact of a high-fat diet in the developing and adult hypermuscular myostatin null mouse. 2015 Skelet Muscle , Band : 5, Seiten : 38 - 38Boehler C , Oberueber F , Stieglitz T , Asplund M Iridium Oxide (IrOx) serves as adhesion promoter for conducting polymers on neural microelectrodes 2015 Neural Engineering (NER), 2015 7th International IEEE/EMBS Conference , Seiten : 410 - 413» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Conducting polymers (CPs) as functional coatings on microelectrodes enable the realization of neural probes with superior electrical properties compared to metallized probes. Besides significantly lower impedance and enhanced charge delivery capacity, CPs further feature the possibility to release drugs from their bulk which can be done exclusively from these materials. Thus the usage of CPs at the neural interface for recording or stimulation of neural tissue is of great interest. A drawback that has however been observed at usage of conducting polymers in vitro and in vivo is the weak adhesion of the polymer to the substrate which ultimately leads to delamination of the coatings. This effect has limited the applicability of polymer coatings on neural probes despite their overall promising potential. In our study we address this gap by introducing Iridium Oxide (IrOx) as adhesion promoter for long-term stabilization of CP films. Exaggerated stressing protocols revealed superior adhesion of the polymer to the rough structure of IrOx and electrochemical measurements indicated unrestricted polymer functionality. With the herein proposed strategy a major obstacle of using conducting polymers at the neural interface could be efficiently targeted and thus applicability of CPs for neural interfaces can be extended in future electrode generations.Johnson GR , Buck TE , Sullivan DP , Rohde GK , Murphy RF Joint modeling of cell and nuclear shape variation. 2015 Mol Biol Cell , Band : 26, Nummer : 22, Seiten : 4046 - 4056Samyn P , Schoukens G , Stanssens D Kaolinite Nanocomposite Platelets Synthesized by Intercalation and Imidization of Poly(styrene-co-maleic anhydride) 2015 MATERIALS , Band : 8, Nummer : 7, Seiten : 4363 - 4388» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung A synthesis route is presented for the subsequent intercalation, exfoliation and surface modification of kaolinite (Kln) by an imidization reaction of high-molecular weight poly(styrene-co-maleic anhydride) or SMA in the presence of ammonium hydroxide. In a first step, the intercalation of ammonolyzed SMA by guest displacement of intercalated dimethylsulfoxide has been proven. In a second step, the imidization of ammonolyzed SMA at 160 degrees C results in exfoliation of the kaolinite layers and deposition of poly(styrene-co-maleimide) or SMI nanoparticles onto the kaolinite surfaces. Compared with a physical mixture of Kln/SMI, the chemically reacted Kln/SMI provides more efficient exfoliation and hydrogen bonding between the nanoparticles and the kaolinite. The kaolinite nanocomposite particles are synthesized in aqueous dispersion with solid content of 65 wt %. The intercalation and exfoliation are optimized for a concentration ratio of Kln/SMI = 70:30, resulting in maximum intercalation and interlayer distance in combination with highest imide content. After thermal curing at 135 degrees C, the imidization proceeds towards a maximum conversion of the intermediate amic acid moieties. The changes in O-H stretching and kaolinite lattice vibrations have been illustrated by infrared and FT-Raman spectroscopy, which allow for a good quantification of concentration and imidization effects.Klatt J , Muehlbacher L , Komnik A Kondo effect and the fate of bistability in molecular quantum dots with strong electron-phonon coupling 2015 Phys Rev B , Band : 91Mohseni F , Mendoza M , Succi S , Herrmann HJ Lattice Boltzmann model for resistive relativistic magnetohydrodynamics. 2015 Phys Rev E , Band : 92, Nummer : 2, Seiten : 023309 - 023309Nystrom A , Thriene K , Mittapalli V , Kern JS , Kiritsi D , Dengjel J , Bruckner-Tuderman L Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms. 2015 Embo Mol Med , Band : 7, Nummer : 9, Seiten : 1211 - 1228Concha-Grana E , Muniategui-Lorenzo S , ... , Reski R , Lopez-Mahia P , Prada-Rodriguez D Matrix solid phase dispersion method for determination of polycyclic aromatic hydrocarbons in moss 2015 JOURNAL OF CHROMATOGRAPHY A , Band : 1406, Seiten : 19 - 26» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung In this work a matrix solid-phase dispersion extraction method, followed by programmed temperature vaporization-gas chromatography-tandem mass spectrometry determination is proposed for the analysis of polycyclic aromatic hydrocarbons (PAHs) in moss samples. A devitalized, cultivated Sphagnum palustre L. moss clone obtained from the “Mossclone” EU-FP7 Project was used for the optimization and validation of the proposed method. Good trueness (84-116%), precision (intermediate precision lower than 11%) and sensitivity (quantitation limits lower than 1.7 ng g(-1)) were obtained. The proposed method was compared with other procedures applied for this complex matrix, achieving a considerable reduction of sample amount, solvent volume and time consumption. The procedure was successfully tested for the analysis of PAHs in exposed moss clone samples for the monitoring of air pollution. Finally, the method was also tested for its suitability in the analysis of PAHs in other moss species as well as a lichen species. (C) 2015 Elsevier B.V. All rights reserved.Haug S , Schnerch D. , Halbach S. , Mastroianni J. , Dumit V , ... , Dengjel J , et al. Metadherin exon 11 skipping variant enhances metastatic spread of ovarian cancer 2015 INTERNATIONAL JOURNAL OF CANCER , Band : 136, Nummer : 10, Seiten : 2328 - 2340» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Metastatic ovarian cancer has a dismal prognosis and current chemotherapeutic approaches have very limited success. Metadherin (MTDH) is expressed in human ovarian cancer tissue and its expression inversely correlates with patients overall survival. Consistent with these studies, we observed MTDH expression in tissue specimens of FIGO Stage III ovarian carcinomas (72/83 cases). However, we also observed this in normal human ovarian epithelial (OE) cells, which raised the question of whether MTDH-variants with functional differences exist. We identified a novel MTDH exon 11 skipping variant (MTDHdel) which was seen at higher levels in ovarian cancer compared to benign OE cells. We analyzed MTDH-binding partner interactions and found that 12 members of the small ribosomal subunit and several mRNA binding proteins bound stronger to MTDHdel than to wildtype MTDH which indicates differential effects on gene translation. Knockdown of MTDH in ovarian cancer cells reduced the amount of distant metastases and improved the survival of ovarian cancer-bearing mice. Selective overexpression of the MTDHdel enhanced murine and human ovarian cancer progression and caused a malignant phenotype in originally benign human OE cells. MTDHdel was detectable in microdissected ovarian cancer cells of some human tissue specimens of ovarian carcinomas. In summary, we have identified a novel MTDH exon 11 skipping variant that shows enhanced binding to small ribosomal subunit members and that caused reduced overall survival of ovarian cancer bearing mice. Based on the findings in the murine system and in human tissues, MTDHdel must be considered a major promalignant factor for ovarian cancer. What’s new? Metastatic ovarian cancer is a deadly malignancy. Its spread through the peritoneum is not well understood but appears to involve distinct molecular mechanisms. Here, in human and murine ovarian cancer cells, overexpression of a newly identified skipping variant in exon 11 of metadherin (MTDHdel) was found to be associated with decreased survival in cancer-bearing mice. Compared with wild-type MTDH, MTDHdel showed stronger binding interactions with 12 members of the small ribosomal subunit and certain mRNA binding proteins. The findings suggest that MTDHdel is an important malignancy-promoting factor and potential therapeutic target in ovarian cancer.Campana F Mihai Orbifold generic semi-positivity: an application to families of canonically polarized manifolds 2015 Ann. Inst. Fourier , Band : 65, Nummer : 2, Seiten : 835 - 861Erlacher M , Strahm B Missing Cells: Pathophysiology, Diagnosis, and Management of (Pan)Cytopenia in Childhood. 2015 Front Pediatr , Band : 3, Seiten : 64 - 64Mueller SJ , Reski R Mitochondrial Dynamics and the ER: The Plant Perspective. 2015 Front Cell Dev Biol , Band : 3, Seiten : 78 - 78Schreiber A , Huber MC , Colfen H , Schiller SM Molecular protein adaptor with genetically encoded interaction sites guiding the hierarchical assembly of plasmonically active nanoparticle architectures. 2015 Nat Commun , Band : 6, Seiten : 6705 - 6705Reski R , Parsons J , Decker EL Moss-made pharmaceuticals: from bench to bedside. 2015 Plant Biotechnol J , Band : 13, Nummer : 8, Seiten : 1191 - 1198Wilde A , Mullineaux CW Motility in cyanobacteria: polysaccharide tracks and Type IV pilus motors 2015 Mol Microbiol , Band : 98, Seiten : 998 - 1001Kebekus S , Greb D , Peternell T Movable curves and semistable sheaves 2015 Int Math Res Notices , Band : 2, Seiten : 536 - 570Boehler C , Stieglitz T , Asplund M Nanostructured platinum grass enables superior impedance reduction for neural microelectrodes 2015 Biomaterials » Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Micro-sized electrodes are essential for highly sensitive communication at the neural interface with superior spatial resolution. However, such small electrodes inevitably suffer from high electrical impedance and thus high levels of thermal noise deteriorating the signal to noise ratio. In order to overcome this problem, a nanostructured Pt-coating was introduced as add-on functionalization for impedance reduction of small electrodes. In comparison to platinum black deposition, all used chemicals in the deposition process are free from cytotoxic components. The grass-like nanostructure was found to reduce the impedance by almost two orders of magnitude compared to untreated samples which was lower than what could be achieved with conventional electrode coatings like IrOx or PEDOT. The realization of the Pt-grass coating is performed via a simple electrochemical process which can be applied to virtually any possible electrode type and accordingly shows potential as a universal impedance reduction strategy. Elution tests revealed non-toxicity of the Pt-grass and the coating was found to exhibit strong adhesion to the metallized substrate.Bostrom M , Thiyam P , Persson C , Parsons D , Buhmann S , Brevik I , Sernelius Bo Non-perturbative theory of dispersion interactions 2015 PHYSICA SCRIPTA , Band : 90, Nummer : 3» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Some open questions exist with fluctuation-induced forces between extended dipoles. Conventional intuition derives from large-separation perturbative approximations to dispersion force theory. Here, we present a full non-perturbative theory. In addition, we discuss how one can take into account finite dipole size corrections. It is of fundamental value to investigate the limits of validity of the perturbative dispersion force theory.Endres D , Perlov E , Maier S , Feige B , Nickel K , Goll P , Bubl E , Lange T , ... , Tebartz van Elst L Normal Neurochemistry in the Prefrontal and Cerebellar Brain of Adults with Attention-Deficit Hyperactivity Disorder. 2015 Front Behav Neurosci , Band : 9, Seiten : 242 - 242Fischer S , Buchleitner A On the derivation of the semiclassical approximation to the quantum propagator 2015 JOURNAL OF MATHEMATICAL PHYSICS , Band : 56, Nummer : 7» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung In order to rigorously derive the amplitude factor of the semiclassical approximation to the quantum propagator, we extend an existing method originally devised to evaluate Gaussian path-integral expressions. Using a result which relates the determinant of symmetric block-tridiagonal matrices to the determinants of their blocks, two difference equations are obtained. The first one allows to establish the connection of the amplitude factor to Jacobi’s accessory equations in the continuous-time limit, while the second one leads to an additional factor which, however, contributes to the final result only in exceptional cases. In order to demonstrate the wide applicability of these difference equations, we treat explicitly the case where the time-sliced Lagrangian is written in generalized coordinates, for which a general derivation has so far been unavailable. (C) 2015 AIP Publishing LLC.Campana F On the h-principle and specialness for complex projective manifolds. 2015 Algebr Geom Topol , Band : 2, Nummer : 3, Seiten : 298 - 314Alexandrov A Open intersection numbers, matrix models and MKP hierarchy 2015 JOURNAL OF HIGH ENERGY PHYSICS , Nummer : 3» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung In this paper we conjecture that the generating function of the intersection numbers on the moduli spaces of Riemann surfaces with boundary, constructed recently by R. Pandharipande, J. Solomon and R. Tessler and extended by A. Buryak, is a tau-function of the KP integrable hierarchy. Moreover, it is given by a simple modification of the Kontsevich matrix integral so that the generating functions of open and closed intersection numbers are described by the MKP integrable hierarchy. Virasoro constraints for the open intersection numbers naturally follow from the matrix integral representation.Biskup T , Sommer M , Rein S , Meyer DL , Kohlstadt M , Wurfel U , Weber S Ordering of PCDTBT revealed by time-resolved electron paramagnetic resonance spectroscopy of its triplet excitons. 2015 Angew Chem Int Edit , Band : 54, Nummer : 26, Seiten : 7707 - 7710Ziletti A , Carvalho A , Campbell DK , Coker DF , Castro Neto AH Oxygen defects in phosphorene. 2015 Phys Rev Lett , Band : 114, Nummer : 4, Seiten : 046801 - 046801Regen W , Hertenstein E , ... , Spiegelhalder K Perfectionistic Tendencies in Insomnia Patients’ Behavior During Psychometric Testing 2015 BEHAVIORAL SLEEP MEDICINE , Band : 13, Nummer : 5, Seiten : 387 - 394» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung According to self-report questionnaire studies, insomnia patients differ from healthy controls with respect to several personality traits. The current study aimed at exploring how these personality traits may translate into behavior. Insomnia patients’ behavior during psychometric testing (n = 163) was investigated in comparison to healthy controls (n = 81), patients with other sleep disorders (n = 80), and patients with obsessive-compulsive disorder (n = 36). In line with our hypotheses, insomnia patients made more additional comments than healthy controls and more corrections than patients with other sleep disorders during sleep-related questionnaire completion. Furthermore, insomnia patients calculated the sum score of a depression questionnaire more frequently than both healthy controls and patients with other sleep disorders. These findings further support the assumption of an altered personality profile in patients with primary insomnia. Future work should aim to elucidate what personality factors these novel behavioral markers may reflect.Fudim R , Mehlhorn J , Berthold T , Weber S , Schleicher E , Kennis JT , Mathes T Photoinduced formation of flavin radicals in BLUF domains lacking the central glutamine. 2015 Febs J , Band : 282, Nummer : 16, Seiten : 3161 - 3174Schuergers N , Nurnberg DJ , Wallner T , Mullineaux CW , Wilde A PilB localization correlates with the direction of twitching motility in the cyanobacterium Synechocystis sp. PCC 6803. 2015 Microbiol-uk , Band : 161, Nummer : Pt 5, Seiten : 960 - 966Gessner M , Schlawin F , Buchleitner A Probing polariton dynamics in trapped ions with phase-coherent two-dimensional spectroscopy 2015 JOURNAL OF CHEMICAL PHYSICS , Band : 142, Nummer : 21» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung We devise a phase-coherent three-pulse protocol to probe the polariton dynamics in a trapped-ion quantum simulation. In contrast to conventional nonlinear signals, the presented scheme does not change the number of excitations in the system, allowing for the investigation of the dynamics within an N-excitation manifold. In the particular case of a filling factor one (N excitations in an N-ion chain), the proposed interaction induces coherent transitions between a delocalized phonon superfluid and a localized atomic insulator phase. Numerical simulations of a two-ion chain demonstrate that the resulting two-dimensional spectra allow for the unambiguous identification of the distinct phases, and the two-dimensional line shapes efficiently characterize the relevant decoherence mechanism. (C) 2015 AIP Publishing LLC.Schiller S Protein Tectons in Synthetic Biology The Expansion of Cellular Functionality Combining Chemical Biology of Small Organic Molecules with Protein Tectons-Unnatural Amino Acids, Protein Based Biohybrid Materials and De Novo Organelles 2015 SYNTHETIC BIOLOGY: CHARACTER AND IMPACT , Seiten : 139 - 154» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The expansion of cellular functions via novel modular building blocks, namely unnatural amino acids, their site-selective genetically encoded cotranslational incorporation into proteins, requires the redesign and expansion of the translational network with additional components, the orthogonal tRNA and tRNA synthetase. At the next level protein tectons (tecton = architectural building block) constitute complex genetically encoded “material libraries” inside the cell. These protein tectons are architectural building blocks allowing for complex supramolecular self-assembly inside the cell, forming cellular compartments or constituting 3D matrix mimicry of the extracellular matrix outside the cell. In addition they form the basis for biohybrid materials in protein/enzyme engineering, nanotechnology and regenerative medicine. The defined modification of protein tectons utilizing chemical biology allows for the selective bioconjugation e.g. of unnatural amino acids, via bioorthogonal chemical reactions introducing novel chemical entities expanding the repertoire of “posttranslational” protein modifications in vitro and in vivo for various applications.Pasternak T , Tietz O , Rapp K , Begheldo M , Nitschke R , Ruperti B , Palme K Protocol: an improved and universal procedure for whole-mount immunolocalization in plants. 2015 Plant Methods , Band : 11, Seiten : 50 - 50Leitner DM Quantum ergodicity and energy flow in molecules 2015 Adv Phys , Band : 64, Nummer : 4, Seiten : 445 - 517Levi F , Mostarda S , Rao F , Mintert F Quantum mechanics of excitation transport in photosynthetic complexes: a key issues review 2015 Rep Prog Phys , Band : 78, Nummer : 8Bae J , Kwek LC Quantum state discrimination and its applications 2015 J. Phys. A: Math. Theor , Band : 48, Nummer : 8, Seiten : 083001 - 083001Taheri H , Stanssens D , Samyn P RHEOLOGICAL CHARACTERIZATION OF A WATERBORNE ORGANIC NANOPARTICLE DISPERSION 2015 APPLIED RHEOLOGY , Band : 25, Nummer : 3, Seiten : 33 - 44» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung Organic nanoparticles of poly(styrene-co-maleimide) or SMI were synthesized in aqueous dispersion with a maximum concentration of 35 wt.% and are favorably applied in industrial coating processes. In order to evaluate the further processability and flow behavior of these nanoparticle dispersions, general rheological characterization under creep, oscillatory and rotational testing was done by applying various shear stresses, shear rates and frequencies on an air-bearing cylindrical rheometer. Creep tests at different stresses show that the nanoparticle dispersions behave like a viscous material. The crossover of G’ and G” according to oscillatory experiments also demonstrates a transition to viscoelastic behavior at high frequency. The sensitivity of shear-viscosity behavior to concentration and temperature of the dispersions has been evaluated. In parallel, the influences of gap size, repeatability and water evaporation have been statistically evaluated and could be successfully controlled. By comparing oscillatory and rotational rheometry data, flow curves under low shear rates were reconstructed.Rassner MP , Hebel JM , Altenmueller DM , Volz S , Feuerstein TJ , Freiman Reduction of epileptiform activity through local valproate-implants in a rat neocortical epilepsy model 2015 Seizure , Band : 30, Seiten : 6 - 13Campana F Représentations linéaires des groupes kählériens: factorisations et conjecture de Shafarevich linéaire. 2015 Compos Math , Band : 151, Nummer : 2, Seiten : 351 - 376Longo J , Yao C , ... , Schiller S , Schaaf P , Jierry L Reversible biomechano-responsive surface based on green fluorescent protein genetically modified with unnatural amino acids 2015 CHEMICAL COMMUNICATIONS , Band : 51, Nummer : 1, Seiten : 232 - 235» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung GFP has been genetically modified at two specific positions of its molecular architecture. These modifications allow its covalent attachment onto PEG brushes grafted on functionalized silicone surfaces. The stretching of this material leads to a reversible decrease of the fluorescence intensity due to stretch-induced forces applying on GFP molecules.Filatov MA , Heinrich E , Busko D , Ilieva IZ , Landfester K , Baluschev S Reversible oxygen addition on a triplet sensitizer molecule: protection from excited state depopulation 2015 Phys Chem Chem Phys , Band : 17, Seiten : 6501 - 6510Carnio EG , Buchleitner A , Gessner M Robust Asymptotic Entanglement under Multipartite Collective Dephasing. 2015 Phys Rev Lett , Band : 115, Nummer : 1, Seiten : 010404 - 010404Cui J , Mintert F Robust control of long distance entanglement in disordered spin chains 2015 New J Phys , Band : 17, Seite : 093014Belyy A , Tabakova I , Lang AE , Jank T , Belyi Y , Aktories K Roles of Asp179 and Glu270 in ADP-Ribosylation of Actin by Clostridium perfringens Iota Toxin. 2015 Plos One , Band : 10, Nummer : 12, Seiten : e0145708 - e0145708Ronsch K , Jagle S , Rose K , Seidl M , Baumgartner F , Freihen V , Yousaf A , Metzger E , Lassmann S , Schule R , Zeiser R , Michoel T , Hecht A SNAIL1 combines competitive displacement of ASCL2 and epigenetic mechanisms to rapidly silence the EPHB3 tumor suppressor in colorectal cancer. 2015 Mol Oncol , Band : 9, Nummer : 2, Seiten : 335 - 354Jalabert R , Weinmann D Scattering approach to scanning gate microscopy 2015 PHYSICA E-LOW-DIMENSIONAL SYSTEMS & NANOSTRUCTURES , Band : 74, Seiten : 637 - 643» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung We present a perturbative approach to the conductance change caused by a weakly invasive scattering potential in a two-dimensional electron gas. The resulting expressions are used to investigate the relationship between the conductance change measured in scanning gate microscopy as a function of the position of a scattering tip and local electronic quantities like the current density. We use a semiclassical approach to treat the case of a strong hard-wall scatterer in a half-plane facing a reflectionless channel. The resulting conductance change is consistent with the numerically calculated quantum conductance. (C) 2015 Elsevier B.V. All rights reserved.Alicki R , Leitner DM Size-Dependent Accuracy of Nanoscale Thermometers. 2015 J Phys Chem B , Band : 119, Nummer : 29, Seiten : 9000 - 9005Nöbauer T , Angerer A , Bartels B , Trupke M , Rotter S , Schmiedmayer J , Mintert F , Majer J Smooth optimal quantum control for robust solid state spin magnetometry 2015 Phys Rev Lett , Band : 115, Seite : 190801Campana F Special manifolds, arithmetic and hyperbolic aspects: a short survey. Rational points, rational curves, and entire holomorphic curves on projective varieties 2015 Amer. Math. Soc. , Seiten : 23 - 52Walschaers M , Mulet R , Wellens T , Buchleitner A Statistical theory of designed quantum transport across disordered networks. 2015 Phys Rev E , Band : 91, Nummer : 4, Seiten : 042137 - 042137Lind C , Dreyer I , Lopez-Sanjurjo EJ , von Meyer K , Ishizaki K , Kohchi T , Lang D , Zhao Y , Kreuzer I , Al-Rasheid KA , Ronne H , Reski R , Zhu JK , Geiger D , Hedrich R Stomatal guard cells co-opted an ancient ABA-dependent desiccation survival system to regulate stomatal closure. 2015 Curr Biol , Band : 25, Nummer : 7, Seiten : 928 - 935Eisenreich W , ... , Bacher A , Weber S Strategy for Enhancement of (13)C-Photo-CIDNP NMR Spectra by Exploiting Fractional (13)C-Labeling of Tryptophan. 2015 J Phys Chem B , Band : 119, Nummer : 43, Seiten : 13934 - 13943Verdeny A , Mintert F Structure-dynamics relation in shaken optical lattices 2015 Phys Rev A , Band : 92, Seite : 033407Karr JR , Williams AH , Zucker JD , Raue A , Steiert B , Timmer J , Kreutz C , Wilkinson S , Allgood BA , Bot BM , Hoff BR , Kellen MR , Covert MW , Stolovitzky GA , Meyer P Summary of the DREAM8 Parameter Estimation Challenge: Toward Parameter Identification for Whole-Cell Models. 2015 Plos Comput Biol , Band : 11, Nummer : 5, Seiten : e1004096 - e1004096Samyn P , Van Nieuwkerke D , Schoukens G , Stanssens D , Vonck L , Van den Abbeele H Synthesis of imidized nanoparticles containing soy oil under various reaction conditions 2015 EUROPEAN POLYMER JOURNAL , Band : 66, Seiten : 78 - 90» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The imidization of poly(styrene-co-maleic anhydride) nanoparticles in presence of soy oil was studied, resulting in the formation of nanoparticles with 20-80 nm diameter with a porous structure allowing to incorporate 50 wt.% soy oil. The reaction efficiency strongly depends on the amount of ammonium hydroxide as imidizing agent, which ratio was varied between 1.01 and 1.60 relatively to the maleic anhydride moieties. Finally, stable aqueous dispersions are obtained with a higher solid content and somewhat higher Zetapotential compared to purely imidized nanoparticles. There are concurring reactions between imidization and coupling of the unsaturated double bonds in soy oil to the ammonolysed maleic anhydride. Therefore, a large amount of non-embedded oil was only observed when the imidization is favoured under high concentrations of ammonium hydroxide. Using spectroscopy and thermal analysis, it is concluded that a complex organized nanostructure is formed between the poly(styrene-co-maleimide) and soy oil, which results in a suppression of T-g evaluated by (temperature-modulated) differential scanning calorimetry. (C) 2015 Elsevier Ltd. All rights reserved.Campana F Th. Rationally connected manifolds and semipositivity of the Ricci curvature. Recent advances in algebraic geometry 2015 London Math. Soc. Lecture Note Ser , Band : 417, Seiten : 71 - 91Petrungaro C , Zimmermann KM , Kuttner V , Fischer M , Dengjel J , Bogeski I , Riemer J The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake. 2015 Cell Metab , Band : 22, Nummer : 4, Seiten : 721 - 733Kirch R , Pinnell R , Hofmann U , Cassel J-C The Double-H Maze: A Robust Behavioral Test for Learning and Memory in Rodents 2015 JOVE-JOURNAL OF VISUALIZED EXPERIMENTS , Nummer : 101Ferlez B , Dong W , ... , Golbeck JH , van der Est A The Effect of Bacteriochlorophyll g Oxidation on Energy and Electron Transfer in Reaction Centers from Heliobacterium modesticaldum 2015 J Phys Chem B , Band : 119, Seiten : 13714 - 13725Sun J , Golbeck J The Presence of the IsiA-PSI Supercomplex Leads to Enhanced Photosystem I Electron Throughput in Iron-Starved Cells of Synechococcus sp PCC 7002 2015 JOURNAL OF PHYSICAL CHEMISTRY B , Band : 119, Nummer : 43, Seiten : 13549 - 13559Pfaumann V , Lang A , Schwan C , Schmidt G , Aktories K The actin and Rho-modifying toxins PTC3 and PTC5 of Photorhabdus luminescens: enzyme characterization and induction of MAL/SRF-dependent transcription 2015 CELLULAR MICROBIOLOGY , Band : 17, Nummer : 4, Seiten : 579 - 594Bohrer C , Pfurr S , Mammadzada K , Schildge S , Plappert L , Hils M , Pous L , Rauch KS , Dumit VI , Pfeifer D , Dengjel J , Kirsch M , Schachtrup K , Schachtrup C The balance of Id3 and E47 determines neural stem/precursor cell differentiation into astrocytes. 2015 Embo J , Band : 34, Nummer : 22, Seiten : 2804 - 2819Lenz L , Hoenderdos M , Prinsen P , Schiessel H The influence of DNA shape fluctuations on fluorescence resonance energy transfer efficiency measurements in nucleosomes. 2015 J Phys-condens Mat , Band : 27, Nummer : 6, Seiten : 064104 - 064104Frank DO , Dengjel J , Wilfling F , Kozjak-Pavlovic V , Hacker G , Weber A The pro-apoptotic BH3-only protein Bim interacts with components of the translocase of the outer mitochondrial membrane (TOM). 2015 Plos One , Band : 10, Nummer : 4, Seiten : e0123341 - e0123341Milton K , Abalo E , ... , Buhmann S , Scheel S Three-body effects in Casimir-Polder repulsion 2015 PHYSICAL REVIEW A , Band : 91, Nummer : 4» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung In this paper we study an archetypical scenario in which repulsive Casimir-Polder forces between an atom or molecule and two macroscopic bodies can be achieved. This is an extension of previous studies of the interaction between a polarizable atom and a wedge, in which repulsion occurs if the atom is sufficiently anisotropic and close enough to the symmetry plane of the wedge. A similar repulsion occurs if such an atom passes a thin cylinder or a wire. An obvious extension is to compute the interaction between such an atom and two facing wedges, which includes as a special case the interaction of an atom with a conducting screen possessing a slit, or between two parallel wires. To this end we further extend the electromagnetic multiple-scattering formalism for three-body interactions. To test this machinery we reinvestigate the interaction of a polarizable atom between two parallel conducting plates. In that case, three-body effects are shown to be small and are dominated by three-and four-scattering terms. The atom-wedge calculation is illustrated by an analogous scalar situation, described in the Appendix. The wedge-wedge-atom geometry is difficult to analyze because this is a scale-free problem. However, it is not so hard to investigate the three-body corrections to the interaction between an anisotropic atom or nanoparticle and a pair of parallel conducting cylinders and show that the three-body effects are very small and do not affect the Casimir-Polder repulsion at large distances between the cylinders. Finally, we consider whether such highly anisotropic atoms needed for repulsion are practically realizable. Since this appears rather difficult to accomplish, it may be more feasible to observe such effects with highly anisotropic nanoparticles.Verdeny A , Mintert F Tunable Chern insulator with optimally shaken lattices 2015 Phys Rev A , Band : 92, Seite : 063615Lin J , Balamurugan D , Zhang P , Skourtis SS , Beratan DN Two-Electron Transfer Pathways. 2015 J Phys Chem B , Band : 119, Nummer : 24, Seiten : 7589 - 7597Jank T , ... , Driever W , Aktories K Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos 2015 NATURE COMMUNICATIONS , Band : 6Yakutkin V , Filatov MA , Ilieva IZ , Landfester K , Miteva T , Baluschev S Upconverting the IR-A Range of the Sun Spectrum using Palladium Tetraaryltetraanthra[2,3]porphyrins 2015 Photoch Photobio Sci Lange T , Buechert M , Baumstark MW , Deibert P , Gerner S , Ryden H , Seufert J , Korsten-Reck U Value of MRI and MRS fat measurements to complement conventional screening methods for childhood obesity. 2015 Jmri-j Magn Reson Im , Band : 42, Nummer : 5, Seiten : 1214 - 1222Antoniou P , Ma Z , Zhang P , Beratan DN , Skourtis SS Vibrational control of electron-transfer reactions: a feasibility study for the fast coherent transfer regime. 2015 Phys Chem Chem Phys , Band : 17, Nummer : 46, Seiten : 30854 - 30866Leitner DM , Buchenberg S , Brettel P , Stock G Vibrational energy flow in the villin headpiece subdomain: master equation simulations. 2015 J Chem Phys , Band : 142, Nummer : 7, Seiten : 075101 - 075101Ditengou F , Mueller A , ... , Palme K , Schnitzler JP , Polle A Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture 2015 NATURE COMMUNICATIONS , Band : 6» Kurzfassung anzeigen « Kurzfassung verbergen Kurzfassung The mutualistic association of roots with ectomycorrhizal fungi promotes plant health and is a hallmark of boreal and temperate forests worldwide. In the pre-colonization phase, before direct contact, lateral root (LR) production is massively stimulated, yet little is known about the signals exchanged during this step. Here, we identify sesquiterpenes (SQTs) as biologically active agents emitted by Laccaria bicolor while interacting with Populus or Arabidopsis. We show that inhibition of fungal SQT production by lovastatin strongly reduces LR proliferation and that (-)-thujopsene, a low-abundance SQT, is sufficient to stimulate LR formation in the absence of the fungus. Further, we show that the ectomycorrhizal ascomycote, Cenococcum geophilum, which cannot synthesize SQTs, does not promote LRs. We propose that the LR-promoting SQT signal creates a win-win situation by enhancing the root surface area for plant nutrient uptake and by improving fungal access to plant-derived carbon via root exudates.Schwab MM , Himmel D , Kacprzak S , Kratzert D , Radtke V , Weis P , Ray K , Scheidt EW , Scherer W , de Bruin B , Weber S , Krossing I [Ni(cod)2][Al(OR(F))4], a Source for Naked Nickel(I) Chemistry. 2015 Angew Chem Int Edit , Band : 54, Nummer : 49, Seiten : 14706 - 14709Filatov MA , Heinrich E , Landfester K , Baluschev S meso-Tetraphenylporphyrin with a -system extended by fusion with anthraquinone 2015 Org Biomol Chem , Band : 13, Seiten : 6977 - 6983
Buchbeiträge Jahre: 2015 | alle anzeigen zurück zur Übersicht aller Publikationen Aktories K , Schmidt G , Lang A Photorhabdus luminescens Toxins TccC3 and TccC5: Insecticidal ADP-Ribosyltransferases that Modify Threonine and Glutamine In : ENDOGENOUS ADP-RIBOSYLATION 2015, Curr. Top. Microbiol. Immunol. , Curr. Top. Microbiol. Immunol., Seiten : 53 - 67, Curr. Top. Microbiol. Immunol.,