Zentrum für Biosystemanalyse
PhD Student with Junior Fellow Jörn Dengjel
Protein dynamics during autophagy analyzed by quantitative mass spectrometry
Autophagy plays an essential role in cell maintenance, differentiation and development. It is thought to be important for the turn-over of whole organelles and long-lived proteins. The aim of this project is to reveal regulation, other subtypes, possible selectivity of autophagy and possible cross-talk between the autophagosomal and proteasomal system. These subjects are investigated by using a combination of techniques including quantitative mass spectrometry (MS)-based proteomics relying on stable isotope labeling by amino acids in cell culture (SILAC) to follow global protein dynamics during autophagy.