Postdoc with External Senior Fellow Juoni Uitto
Verónica Dumit obtained her First Degree in Biotechnology in 2005 at the University of Rosario, Argentina, where she also completed her Doctoral Thesis in 2010, at the Department of Molecular Biology. Part of her graduate studies was done at the University of Bayreuth, Germany, in the Department of Computational Biochemistry, in the frame of a collaboration with the University of Rosario. In June 2011, she joined the group of Dr. Jörn Dengjel as postdoctoral fellow in the School for Life Sciences-LifeNet at FRIAS.
Systemic scleroderma is a fibrotic disease characterized by abnormal and excessive deposition of collagen and other extracellular matrix components in various tissues. Typical of this disorder is the presence of extracellular matrix producing fibroblasts in the affected organs displaying an activated phenotype. We employ stable isotope labeling by amino acids in cell culture (SILAC), a metabolic labeling strategy, which allows mass spectrometric comparison of different cell states or types to unravel proteomic changes of the extracellular matrix and intracellular protein composition between normal and affected skin fibroblasts. Using this global, hypothesis-free approach we are able to quantify several thousand proteins and to detect their diferential expression levels in scleroderma fibroblasts. By bioinformatics analyses, we intend to determine the underlying connection between protein expression and intracellular pathways being altered in scleroderma and possibly being involved in pathogenesis.
- Dumit, VI; Cortez, N and Ullmann, GM. (2011) Distinguishing two groups of flavin reductases by analyzing the protonation state of an active site carboxylic acid. Proteins, 79: 2076–2085.
- Dumit, VI; Bortolotti, A; Cortez, N and Ullmann, GM. (2011) NADP(H) binding to Ferredoxin-NADP(H)-reductase from Rhodobacter capsulatus, and the contribution of the conserved glutamate in the active site to the process. Flavins and Flavoproteins 2011. In press.
- Dumit, VI; Essigke, T; Cortez, N and Ullmann, GM. (2010) Mechanistic insights into ferredoxin catalysis involving the conserved glutamate in the active site. J. Mol. Biol., 397, 814-25.
- Dumit, VI; Bortolotti, A; Carrillo, N; Cortez, N; Jouanneau, Y and Medina, M. (2008) Rhodobacter capsulatus ferredoxinVI and ferredoxin(flavodoxin)-NADP(H) oxidoreductase are redox partners in vitro. In Flavins and Flavoproteins 2008. (S. Frago, C. Gómez-Moreno, M. Medina, eds.) Zaragoza University Press, 107-112.